Molecular Biology

• Northern blot
• RT-PCR
• Primers for RT-PCR
• Quantitative RT-PCR
• Primers for Quantitative RT-PCR
• Western blot for phosphoprotein samples

Mouse Protocols

• Alcian blue - Alizarin red skeletal staining
• Alcian blue cartilage staining
• Immunohistochemistry on mouse sections
• In situ hybridization on mouse cryostat sections
• In situ hybridization on mouse paraffin sections
• Mallory's tetrachrome
• Whole mount in situ hybridization on mouse embryos

Fly Protocols

• Abdominal cuticle preparations
• Cuticle preparations
• Fly whole mount immunostaining
• Mounting of adult wings
• Wing disc fixation and immunostaining

• Primers for quantitive RT-PCR

Cell Biology

• Cell Immunostaining
• Dual luciferase
• Immunostaining of dissociated Drosophila embryonic cells
• Pulse & chase labeling with 35S-methionine
• Synchronization of cell division

Lise Zakin Geissert coordinated the update and posting of these protocols.

Last Updated: April 2017